An ELISA test may be used to diagnose: HIV, which causes AIDS.
An ELISA also called EIA, is a test that detects and measures antibodies in your blood. Antibodies are proteins that your body produces in response to harmful substances called antigens.
Amylase, also called Ptyalin, which is capable of breaking down starch into simpler sugars such as maltose and dextrin that can be further broken down in the small intestine is the Enzyme present in Saliva.
The earliest historic evidences of use of metal are of Chalcolithic Age or Stone-Copper Age, which covered the period from 1800 - 800 BC. This period was by the use of Copper as well as stone.
Hence, the first metal to be used in India is Copper.